# #——回声= FALSE,结果= "隐藏 "---------------------------------------------- knitr: opts_chunk美元集(错误= FALSE,警告= FALSE,消息= FALSE, fig.retina = 0.75,作物= NULL)图书馆(BiocStyle) # #——图书馆,回声= FALSE ------------------------------------------------------ 库(cytomapper ) ## ---- 消息= FALSE ----------------------------------------------------------- 库(HDF5Array) #定义输出目录cur_dir < - getHDF5DumpDir () path.to.images < -系统。文件("extdata"， package = "巨细胞程序")图像。list <- loadImages(path.to. list)images, pattern = "mask.tiff"， on_disk = TRUE, h5FilesPath = cur_dir) #显示列表图像。缩放图像。list <- scaleImages(image. list)List, value = 2^16 - 1) image。列表E34_mask美元 ## ----------------------------------------------------------------------------- 数据(“pancreasImages”)pancreasImages_onDisk < - CytoImageList (pancreasImages on_disk = TRUE, h5FilesPath = cur_dir) #图像对象pancreasImages E34_imc # HDF5Array对象pancreasImages_onDisk E34_imc #美元种子HDF5Array对象的种子(pancreasImages_onDisk E34_imc美元)#在内存大小格式(object.size (pancreasImages),单位=“汽车”)格式(object.size (pancreasImages_onDisk),单位= "汽车 ") ## ----------------------------------------------------------------------------- pancreasImages_inMemory < - CytoImageList (pancreasImages_onDisk on_disk = FALSE) #比较原始图像数据表示相同(as.list (pancreasImages_inMemory) as.list (pancreasImages )) ## ---- 完整性检查,回声= FALSE ------------------------------------------------- stopifnot(相同(as.list (pancreasImages),as.list (pancreasImages_inMemory ))) ## ----------------------------------------------------------------------------- # 大小的对象在内存中格式(object.size (pancreasImages_onDisk),单位=“汽车”)#大小的对象在磁盘上kB file.info (paste0 (cur_dir, " / E34_imc.h5 ")("大小"]/ 1000 pancreasImages_norm < -正常化(pancreasImages_onDisk)种子(pancreasImages_norm E34_imc美元)#大小的对象在内存中格式(object.size (pancreasImages_norm),单位=“汽车”)#大小的对象在磁盘上kB file.info (paste0 (cur_dir " / E34_imc.h5 ")) / 1000,“大小” ## ----------------------------------------------------------------------------- cur_Images1 < - pancreasImages_onDisk cur_Images2 < - getChannels (pancreasImages_onDisk, 2) channelNames (cur_Images2) < -“CD99_2 setChannels (cur_Images1, 1) < - cur_Images2格式(object.size (cur_Images1),单位= "汽车 ") ## ----------------------------------------------------------------------------- channels1 < - getChannels (pancreasImages_onDisk 1:2) channels2 < - getChannels (pancreasImages_onDisk 3:4) dir.create(文件。Path (cur_dir， "test")) cur_path_2 <- file。路径(cur_dir“测试”)channels3 <——mergeChannels (channels1、channels2 h5FilesPath = cur_path_2)种子(channels3 E34_imc美元)# #——sessionInfo回声= FALSE -------------------------------------------------- sessionInfo ()