# #——风格,回声= FALSE,结果= '飞机 '-------------------------------------------------------- BiocStyle:减价()选项(宽度= 100,max.print = 1000) knitr:: opts_chunk设置(eval = as.logical (Sys美元。采用“KNITR_EVAL”,“真正的”)),缓存= as.logical (Sys。采用“KNITR_CACHE”,“真正的”)),错误= FALSE) # #——加载数据 ------------------------------------------------------------------------------------ 图书馆(“气道”)的数据(气管)# #——玩 ----------------------------------------------------------------------------------------- 库(“气道”)数据(气管)se < -气道se # #------------------------------------------------------------------------------------------------- 库(DESeq2) dds < - DESeqDataSet细胞(se设计= ~ +敏捷 ) ## ------------------------------------------------------------------------------------------------- dds敏捷< relevel (dds敏捷美元,美元“untrt ") ## ------------------------------------------------------------------------------------------------- dds < - DESeq (dds ) ## ------------------------------------------------------------------------------------------------- ( res (dds)) # # < -结果------------------------------------------------------------------------------------------------- mcols (res use.names = TRUE ) ## ------------------------------------------------------------------------------------------------- 总结(res) # #——plotcounts fig.width = 5,fig.height = 5 -------------------------------------------------------- topGene < - rownames (res) [which.min (res padj美元)]plotCounts (dds,基因= topGene intgroup = c(“敏捷 ")) ## ---- sessionInfo ---------------------------------------------------------------------------------- sessionInfo ()